Therefore, it is crucial to have an interface to connect the LC outlet to the MS inlet that can efficiently transfer the LC mobile phase to gas and at the same time ionize the analytes. The gaseous compounds are ionized through various methods such as electron ionization (EI) and chemical ionization (CI). This high energy ionization fragments the. Liquid chromatography (LC) coupled to mass spectrometry (MS) is today a well- established analytical technique (LC–MS) that, in the last few decades, has opened the door to many challenging applications. MS is undoubtedly the most powerful detector that can exploit the separation capability of an. The analysis of biomolecules by mass spectrometry (MS) put rather severe constraints on the ionization method, as far as its ability to produce intact biomolecular ions (without uncontrollable fragmentation) is concerned. It generally consists of a LC separating system, a mass analyzer and the LCMS interface API unit.
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